Annals of Cardiac Anaesthesia Annals of Cardiac Anaesthesia Annals of Cardiac Anaesthesia
Home | About us | Editorial Board | Search | Ahead of print | Current Issue | Archives | Submission | Subscribe | Advertise | Contact | Login 
Users online: 3390 Small font size Default font size Increase font size Print this article Email this article Bookmark this page


    Advanced search

    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  

    Article Tables

 Article Access Statistics
    PDF Downloaded261    
    Comments [Add]    

Recommend this journal


Table of Contents
Year : 2022  |  Volume : 25  |  Issue : 4  |  Page : 399-407
Intraoperative blood collection without fluid replacement for cardiac surgery – A retrospective analysis

Department of Anesthesiology, University of Michigan, USA

Click here for correspondence address and email

Date of Submission25-Mar-2021
Date of Decision03-Aug-2021
Date of Acceptance12-Sep-2021
Date of Web Publication10-Oct-2022


Background: Transfusion rates in cardiac surgery are high.
Aim: To determine if intraoperative autologous blood removal without volume replacement is associated with fewer homologous blood transfusions without increasing acute kidney injury.
Setting and Design: Retrospective, comparative study.
Materials and Methods: Adult patients undergoing cardiac surgery, excluding those who underwent ventricular assist device surgery, heart transplants, or cardiac surgery without cardiopulmonary bypass were excluded, who had 1–3 units of intraoperative autologous blood removal were compared to patients without blood removal for determination of volume replacement, vasopressor support, acute kidney injury, and transfusions.
Results: Autologous blood removal was associated with fewer patients receiving homologous transfusions: intraoperative red cell transfusions fell from 75% (Control) to 48% (1 unit removed), 40% (2 units), and 30% (3 units), P < 0.001. Total intraoperative and postoperative homologous RBC units transfused were lower in the blood removal groups: median (interquartile range) 3 (1, 6) in Control patients and 0 (0, 2), 0 (0, 2) and 0 (0, 1) in the 1, 2, and 3 units removed groups, P < 0.001. Similarly, plasma, platelet, and cryoprecipitate transfusions decreased. After adjustment for confounders, increased amounts of autologous blood removal were associated with increased intravenous fluids, only when 2 units were removed, and trivially increased vasopressor use. However, it was not associated with acidosis or acute kidney injury.
Conclusions: Intraoperative autologous blood removal without volume replacement of 1–3 units for later autologous transfusion is associated with decreased homologous transfusions without acidosis or acute kidney injury.

Keywords: Acute kidney injury, autologous blood removal, cardiac surgery, transfusion

How to cite this article:
Vance JL, Irwin L, Jewell ES, Engoren M. Intraoperative blood collection without fluid replacement for cardiac surgery – A retrospective analysis. Ann Card Anaesth 2022;25:399-407

How to cite this URL:
Vance JL, Irwin L, Jewell ES, Engoren M. Intraoperative blood collection without fluid replacement for cardiac surgery – A retrospective analysis. Ann Card Anaesth [serial online] 2022 [cited 2022 Dec 6];25:399-407. Available from:

   Introduction Top

Blood transfusion with cardiac surgery accounts for 20% of transfusions in the United States.[1] While restrictive transfusion thresholds have been shown to produce equivalent outcomes as liberal thresholds, the overall transfusion rate remains high.[2] Transfusions are not benign. They are associated with increased morbidity and mortality.[3],[4] The hemodilution resulting from crystalloid priming of the cardiopulmonary bypass (CPB) circuit represents a major risk factor for blood transfusions. While several techniques are available to limit hemodilution, such as retrograde autologous priming and high-volume ultrafiltration, these may be insufficient to prevent transfusions. Additionally, the abnormal conditions of blood subjected to the various components of the CPB circuit can lead to platelet dysfunction and destruction.[5] The resulting thrombocytopenia or functional thrombocytopenia contributes to postoperative coagulopathy and hemorrhage, with frequent need for erythrocyte (RBC) and platelet transfusions. To minimize the need for transfusions and their associated risk of infection, acute lung injury, and immunomodulation, some physicians use intraoperative blood collection.[6],[7],[8],[9],[10],[11],[12],[13] After induction of general anesthesia, blood is sterilely removed from the patient and stored in bags containing CPDA as a preservative and anticoagulant.[8],[9],[12]

When RBC are removed for intraoperative blood collection, platelets and clotting factors are also captured for reinfusion after discontinuation of CPB. Putatively, this technique conserves blood by decreasing postoperative coagulopathy and the need for transfusions by providing a sufficient quantity of fresh platelets and clotting factors to replace the ones activated or consumed during CPB.[14] However, platelet function was adversely affected by storage in CPDA[15] and did not improve thromboelastography values after CPB.[16] Studies of autologous blood removal primarily used crystalloid or colloids to replace the blood volume [acute normovolemic hemodilution (ANH)].[9],[15],[17] This can lead to hemodilution of platelets, clotting factors, and RBC and increase the risk of homologous RBC transfusion to prevent or treat low oxygen delivery or hemodynamic instability. Instead of crystalloid or colloid infusions to maintain intravascular volume and hemodynamics, hemodynamics can also be maintained with vasopressors. The use of vasopressors and minimizing volume administration [autologous blood removal without fluid replacement (AWOF)] is designed to limit hemodilution and the need to transfuse homologous RBC.

We hypothesized that AWOF would be associated with decreased blood transfusion requirements in a dose-dependent manner. We further hypothesized, as secondary outcomes, that AWOF would be associated with increased vasopressor requirements, no differences in metabolic (acid–base) parameters, no difference in rate of acute kidney injury (AKI), and higher hematocrit and platelet levels on arrival in the intensive care unit.

   Methods Top


This study was approved by the institutional review board, which waived informed consent as it was a retrospective analysis and it was conducted in accordance with the ethical standards of the Helsinki Declaration of 1975 as revised in 2000. STROBE guidelines were used. All adult patients (age ≥18 years) undergoing cardiac surgery between Jan 31, 2007 and Nov 6, 2013 were included in the study. Patients undergoing ventricular assist device surgery, heart transplants, or cardiac surgery without cardiopulmonary bypass were excluded.

Patient management

Fluids (including infusions and cardioplegia, but excluding piggyback and push medicines), blood products, and vasopressors administered by the anesthesiologist or the perfusionist are recorded in the shared electronic anesthetic record (Centricity, GE Healthcare, Chicago, IL) and were abstracted from it. Laboratory values were extracted from the institutional data warehouse. AKI was defined using Kidney Disease: Improving Global Outcomes (KDIGO) stages: creatinine increase >0.3 mg/dL over 48 hours or to 150% of baseline by day 7 was stage 1; creatinine to >200% of baseline was stage 2, and >300% of baseline was stage 3. Urine output was not included in our outcome definition. Hematocrit levels were determined preoperatively, postinduction before blood was removed, postblood removal but prebypass, and 30 minutes after arrival in the ICU. The lowest intraoperative arterial pH and bicarbonate and highest lactic acid level were used as measures of worst metabolic derangement.

Patients routinely received general anesthesia with midazolam, fentanyl, propofol, and a neuromuscular blocker for induction. Etomidate was rarely used. In addition to routine EKG and pulse oximetry, patients had an arterial line and either a central venous catheter or pulmonary artery catheter. Mechanical ventilation with VT = 6–8 mL/kg of predicted body weight, with rate adjusted to achieve normocarbia was used. PreCBP, intravenous fluids, usually lactated ringer's solution, were minimized. Hetastarch was not used. Vasopressors were used as necessary to maintain mean arterial pressure >65 mmHg. Bolus ephedrine and phenylephrine and phenylephrine and norepinephrine infusions were used to support hemodynamics. As needed, vasopressin or epinephrine was used. After establishment of central venous access, an antifibrinolytic infusion was started and continued until shortly before leaving the operating room. Initially, aprotinin (2,000,000 KIU load, followed by infusion at 500,000 KIU/hour for the duration of the operation and a pump prime of 2,000,000 KIU) was used. After its removal from the market, we used aminocaproic acid (70 mg/kg load followed by infusion at 30 mg/kg/hr for the duration of the operation). Tranexamic acid was used when aminocaproic acid was in shortage (8 mg/kg load followed by infusion at 4 mg/kg/hr for the duration of the operation. If creatinine was 1.6-3.3 mg/dL, the tranexamic acid infusion was 3 mg/kg/hour. If creatinine was 3.4-6.6 mg/dL, the infusion was 2 mg/kg/hr. For creatinine >6.6. mg/dL or receipt of dialysis, the infusion was 1 mg/kg/hr. Additionally, 0.6 mg/kg load was added to the bypass circuit.) The decision for removal and amount of autologous blood removal was decided jointly by the surgeon and anesthesiologist. After preparation with chlorhexidine or povidone-iodine, autologous blood was aseptically removed via the central venous access and stored in bags prefilled with CPDA anticoagulant. Bags were not routinely weighed but filled based on visual inspection. Blood was stored at room temperature and gently agitated before being transfused. Prior to cardiopulmonary bypass, heparin 300 U/kg was administered intravenously. The CPB circuit was primed with 900 – 1100 mL of fluid, usually PlasmaLyte. If autologous priming was not used, an additional 300 – 500 mL of fluid was added to the bypass circuit. These volumes were recorded on the anesthetic record and included in the fluid analyses. Mild (32-35°C) or moderate (28-31°C) hypothermia was employed on bypass, with sweeps adjusted to maintain PaCO2 = 40 mmHg.[18] Deep (≤25°C) hypothermia was used with circulatory arrest.[18] Additional heparin doses were given to keep the activated clotting time >400 sec. After separation from CPB, heparin was reversed with protamine (1-1.3 mg per 100 units of initial heparin dose) and confirmed by ACT returning to baseline values. Additional doses of protamine could be given if the ACT was elevated. Heparin concentration assays were not used. After reversal of heparin, the autologous blood was transfused. Physical measures of thrombosis, such as thromboelastography, were not employed. RBC transfusions were based on hematocrit levels (over the study period, the transfusion trigger for RBC decreased from 22-24% to ~18%) and clinical judgment, which included hemodynamics. Plasma, platelet, and cryoprecipitate transfusions were based on clinical judgment, inspection of the surgical field, and, when available, laboratory tests.

Power analysis

Based on current practice, we assumed that 20% of patients have no autologous blood removed and that intraoperative transfusion rate is 20%, then to find a 3% change, an amount for which we would consider changing our practice, in the transfusion rate to 17 or 23% in patients who have any autologous blood removed, with alpha = 0.05 and power = 0.8, would require 4000 subjects.

Statistical analysis

Baseline characteristics were described with means (standard deviations), medians (interquartile range), frequency, and percentages. Differences in categories were tested using one-way ANOVA, the Kruskal–Wallis test, or the Chi-square test. To determine the independent associations of autologous blood removal with outcomes, we used Akaike Information Criteria based linear and logistic regressions. We further analyzed the data using propensity matching. Here, nonparsimonious binary logistic regression using sex, age, ASA class, emergency status, surgery type, year of surgery, type of antifibrinolytic used, height, weight, body mass index, body surface area, platelet count, INR, creatinine, and postinduction hematocrit value was used to calculate a propensity to be in the AWOF group. AWOF patients were matched to Control patients by similar propensity scores using a nearest neighbor greedy algorithm. The match was considered successful if all variables had standardized differences <10%. All analyses were performed with R version 2.14.2 (R Foundation for Computing, Vienna, Austria). Linear regression results are presented as estimate B (95% confidence interval) and logistic regressions as adjusted odds ratio (aOR) (95% confidence interval). As the dose-response (outcome associated with the number of autologous blood units removed) might be nonlinear or have a threshold, we analyzed number of autologous units as a categorical variable in all regressions.

Sensitivity analysis

The main analyses were done adjusting for albumin volume in a 3:1 ratio for 5% and 15:1 ratio for 25% as is traditionally considered.[19] As more recent research has suggested that equal volumes of albumin and crystalloid produce equal expansions of blood volume,[20] we did sensitivity analyses of all regressions using unadjusted volumes.

   Results Top

We studied 2809 patients, 61 ± 15 years old, 1874 (67%) male, with body mass index 29.5 ± 6.3 kg/m2. The preoperative hematocrit was 39.0 ± 5.4%. Five hundred ninety-six patients (21%) had no blood removed (Control group), whereas 482 (17%), 1257 (45%), and 474 (17%) AWOF patients had 1, 2, or 3 units of blood removed, respectively. AWOF patients had higher preoperative hematocrits, but lower creatinine levels and platelet counts [Table 1]. After anesthesia induction and before blood removal, AWOF patients had higher hematocrits than the Control group, but the postinduction hematocrit did not differ within the AWOF group by number of units removed [Table 1].
Table 1: Baseline and operative characteristics. Intravenous fluids adjusted and CPB volume adjusted has the administered volume adjusted for the volume of albumin given (3:1 for 5% and 15:1 for 25% albumin)

Click here to view

Processes of care

Intravenous fluid administration was less in AWOF group with one unit removed compared to Control patients. Only when three units were removed, was intravenous fluid administration greater [Table 1]. The number of boluses of vasopressor doses was statistically, but not clinically, significantly higher in the AWOF groups (median 9 interquartile range (4, 14) for control group, 10.5 (5,17), 10 (5,16), and 13 (8,19) for AWOF = 1, 2, and 3, respectively, P < 0.001). Total phenylephrine dose was greater, but epinephrine and norepinephrine doses were less in the AWOF groups [Table 2]. While there were slight differences in nadir intraoperative pH and bicarbonate among the four groups, overall, there was no trend between pH, bicarbonate, and groups (P = .744 and. 128, respectively). Similarly, while lactic acid levels were lower in AWOF patients with one (2.6 ± 1.4 mmol/L) and two (3.0 ± 1.8 mmol/L) units removed compared to Control (3.6 ± 2.6 mmol/L), P < .001 and. 005, respectively, there was no overall trend in lactic acid levels with the number of blood units removed (P = .410) [Table 3].
Table 2: Vasoactive medications

Click here to view
Table 3: Transfusions and other outcomes

Click here to view

By multivariable linear regression, AWOF patients had similar or slightly better nadir pH, nadir bicarbonate, and peak lactic acid levels then Control patients [Table 4]. Using multiple linear regression to adjust for other demographics, preoperative laboratory values, types of surgery, and antifibrinolytics, AWOF was associated with slightly greater amounts of ephedrine and phenylephrine but lesser amounts of epinephrine, norepinephrine, or vasopressin [Table 4]. There was no difference in the amounts of intravenous or CPB fluids [Table 4].
Table 4: Linear regression showing the association among processes of care, intermediate outcomes, and the number of autologous blood units removed and patients with no autologous blood removed (Control group). Regressions used the adjusted intravenous fluids and cardiopulmonary bypass volume adjusted for the volume of albumin given (3:1 for 5% and 15:1 for 25% albumin). Any transfusion is sum of intraoperative and postoperative

Click here to view


Overall, 1322 (47%) of patients were transfused RBC intraoperatively and 1425 (51%) at any time. We found that there was a decrease in the proportion of patients given intraoperative RBC transfusions as the number of autologous units increased: 75% (0 units) to 48% (1 unit), 40% (2 units), and 30% (≥3 units), P < 0.001. We also found similar decreases in plasma and platelet transfusions from 53% to 19%, P < .001 and from 57% to 23%, P < .001, respectively, but not in cryoprecipitate [Table 3]. After we adjusted for other factors associated with RBC transfusion, patients with AWOF were less likely to be given RBC, plasma, and platelets and AWOF was associated with fewer units of RBC, plasma, and platelets transfused [Table 5]. However, the number of cryoprecipitate units transfused was decreased only for patients with three units removed. Any (intraoperative + postoperative) homologous transfusions were similarly decreased by AWOF [Table 3] and [Table 5].
Table 5: Adjusted odds ratio in patients with 1, 2, or 3 units of autologous blood removed compared to patients who had no autologous blood removed (Control group). Regressions used the adjusted intravenous fluids and cardiopulmonary bypass volume adjusted for the volume of albumin given (3:1 for 5% and 15:1 for 25% albumin). Any transfusion is sum of intraoperative and postoperative

Click here to view

Postoperatively, first ICU hematocrits were slightly lower in Control Group and AWOF = 1 compared to AWOF Groups 2 and 3: (Control) 27.5 ± 4.4% versus (Group 1) 27.1 ± 4.3%, versus (Group 2) 28.1 ± 4.0%, versus (Group 3) 28.3 ± 3.9%, respectively, P < .001. After adjusting for other factors, AWOF was associated with slightly lower or similar hematocrit levels on ICU arrival [B = -0.9 (-1.4,-0.4), P < .001; B = -0.3 (-0.8,0.1), P = 0.120; and B = -0.2 (-0.8,0.4) P = 0.480. Platelet counts at ICU arrival were lower in the AWOF groups [Table 2] but after adjustment, only AWOF of three units was associated with lower counts (B = -20 (-27,-13), P < 0.001 [Table 4].

Other outcomes

AWOF patients with two or three units removed had slightly lower rates of KDIGO stage 1 or 3 AKI [Table 3]. However, after using logistic regression, AWOF was not associated with AKI [Table 5]. Reexploration for hemorrhage did not differ among groups [Table 3].

Sensitivity analyses

When we repeated the regressions using actual intravenous and cardiopulmonary fluids instead of adjusting for the putative greater volume expansion attributed to albumin, we had similar results [Supplementary Table 1] and [Supplementary Table 2]. In particular, AWOF was still associated with lower odds of transfusion and fewer units when transfused. Compared to Control Group, AWOF of 1, 2, or 3 units was associated with 2 (1,3), 3 (2, 3), or 5 (3,6) fewer RBC units transfused, respectively; all P < 0.001.

Propensity score matching

When we used propensity scores to match patients with AWOF to Control patients, we had 488 well-matched pairs (all standardized differences <10%). In particular, the postinduction hematocrit was 31.7 ± 5.8% in the Control group versus 31.6 ± 5.7% in the AWOF group, P = .883, standardized difference = 1.8%. Of the 488 AWOF patients, 135 had one, 295 had two, and 58 had three units removed. Similar to the traditional analysis, AWOF patients were more likely to receive vasopressor boluses, more phenylephrine but less norepinephrine, and slightly more fluid adjusted for albumin dose [Supplementary Table 3]. In these paired patients, AWOF patients were less likely to receive intraoperative homologous blood transfusions (75 vs. 53%, P < .001 for RBC; 53 vs. 34%, P < .001 for plasma; 57 vs. 39%, P < .001 for platelets), but had similar rates of cryoprecipitate [Supplementary Table 3]. These differences persisted through the postoperative period. Despite this higher transfusion amount, Control patients did not have higher postoperative hematocrits [Supplementary Table 3].

   Discussion Top

We found that we were successful at removing 1 – 3 units of blood for later transfusion with minimal, if any, increase in intravenous fluids and only small increases in vasopressor requirements. This amount of intraoperative blood collection was associated with fewer transfusions and was well tolerated with no increase in lactic acid, decrease in bicarbonate concentrations or pH, or increase in AKI.

Importantly, we found that AWOF was not associated with AKI, which is similar to one study of ANH, but different than another ANH study, which found lower (28.2% v 24.1%, P < .001) rate of AKI in patients with autologous blood transfusions.[6],[7] While transfusion in cardiac surgery has been associated with AKI,[21] unlike Goldberg et al.'s study,[7] we did not find autologous blood to be associated with a lower AKI rate. Our study differs by removing blood without replacing the lost volume, which may contribute to a fall in cardiac output and renal blood flow. However, compared to ANH, it maintains hematocrit and thus may produce similar oxygen delivery.

We also found that despite fewer RBC transfusions, the change in hematocrit from postinduction to ICU was similar in the propensity matched groups. Our findings of fewer RBC, plasma, and platelet transfusions are similar to studies that used ANH.[6],[8],[9],[10],[11] Given the small numbers of patients who received cryoprecipitate, we may have been underpowered to find a benefit. Platelet count on ICU arrival was lower in the third unit AWOF group [Table 3]; but this was not associated with increased rate of reexploration for hemorrhage [Table 3].

AWOF was associated with a 33-36% lower odds ratio of receiving homologous blood transfusions per unit of blood removed [Table 5]. Our findings of decreased autologous transfusions in AWOF is similar to both prospective randomized and retrospective observational studies and one meta-analysis that found that ANH reduces transfusions.[6],[8],[9],[10],[11] Its similarity is obvious that larger amounts of autologous transfusions were associated with lesser transfusion rates. Our results differ from two studies that found no benefit from ANH.[12],[13] While the volume of autologous blood removed in one study was relatively smaller (1 unit),[13] the volume in the other study was larger [1,099 ± 333 ml (range, 430–1900 ml)].[12] It is possible that large volume removal with fluid replacement leads to excessive hemodilution such that subsequent transfusion is necessary. Other studies of autologous transfusion did not provide information on vasopressors, making comparisons difficult.[22],[23],[24]

AWOF attempts to balance the benefits of autologous blood transfusion with the risks of hypovolemia. Larger volumes of autologous blood result in a higher hematocrit and may proportionally decrease platelet and plasma transfusions by providing fresh platelets and clotting factors postbypass when they are needed. However, larger volumes of autologous blood removal increase the risk of hypovolemia, need for vasopressors, and organ hypoperfusion and injury. Administration of intravenous fluids to maintain normovolemia (ANH) leads to hemodilution, anemia, and the potential for organ dysfunction. In particular, anemia on CBP is associated with AKI and mortality – probably from the decreased oxygen carrying capacity of the resultant anemic blood.[21],[25] AWOF by minimizing hemodilution should lead to a higher oxygen carrying capacity, but its effects on oxygen delivery need further study.

Our study extends those findings to the use of AWOF and shows that AWOF is achievable and has no evidence of perfusion deficits as measured by acid–base balance or AKI. Future studies should compare AWOF to ANH, as AWOF should produce less hemodilution and may lead to fewer transfusions. Additionally, the lesser blood volume may contribute to decreased hemorrhage.[26]

There are several limitations to this study. First, this is a single center study and individual transfusion practices may not be generalizable to other institutions. Multi-center study is needed to confirm our finding. Second, as this was not a blinded study and there was no transfusion protocol, decisions to order homologous blood may have been based, at least in part, on the lack of autologous blood. This bias would create an apparent benefit to transfusion rates in AWOF group. We are also limited in that fluid intake did not include piggyback and push medicines. While typically similar across cases, their absence from fluid intake may have created a small unknown bias. Furthermore, we did not have information on preoperative anticoagulant and antiplatelet use or on use of retrograde autologous priming of the bypass circuit. If use was unbalanced across groups, it may have introduced bias. Both the total population and the propensity matched groups may have unknown confounders that influenced the decision to remove or not remove blood, and this might bias our results. Prospective randomized studies are suggested to overcome these limitations. Finally, while our study had fewer patients than desired by the power analysis, we were able to find a clinically and statistically significant decrease in transfusions in patients who had blood removed without fluid replacement, due to a larger-than-expected effect size. We also had a higher transfusion rate than estimated for the power calculation, however our intra- and post-operative transfusion rates (32% for three units removed to 79% for none) is similar to or better than a recent trial comparing restrictive (52.3%) versus liberal (72.6%) transfusion criteria.[2] The main strengths of this study are that we included patients undergoing a variety of cardiac procedures, not just coronary artery bypass grafts, and that we found similar results with both traditional logistic regression and propensity matching analysis.

In conclusion, we found that AWOF of 1-3 units for later autologous transfusion is associated with decreased homologous transfusions without acidosis or AKI.

Financial support and sponsorship

Supported by departmental and university funds.

Conflicts of interest

There are no conflicts of interest.

   References Top

Whitson BA, Huddleston SJ, Savik K, Shumway SJ. Bloodless cardiac surgery is associated with decreased morbidity and mortality. J Card Surg 2007;22:373-8.  Back to cited text no. 1
Mazer CD, Whitlock RP, Fergusson DA, Hall J, Belley-Cote E, Connolly K, et al. Restrictive or liberal red-cell transfusion for cardiac surgery. N Engl J Med 2017;377:2133-44.  Back to cited text no. 2
Engoren MC, Habib RH, Zacharias A, Schwann TA, Riordan CJ, Durham SJ. Effect of blood transfusion on long-term survival after cardiac operation. Ann Thorac Surg 2002;74:1180-6.  Back to cited text no. 3
Koch CG, Li L, Sessler DI, Figueroa P, Hoeltge GA, Mihaljevic T, et al. Duration of red-cell storage and complications after cardiac surgery. N Engl J Med 2008;358:1229-39.  Back to cited text no. 4
Wahba A, Black G, Koksch M, Rothe F, Preuner J, Schmitz G, et al. Cardiopulmonary bypass leads to preferential loss of activated platelets. A flow cytometric assay of platelet surface antigens. Eur J Cardiothorac Surg 1996;10:768-73.  Back to cited text no. 5
Zhou Z-F, Jia X-P, Sun K, Zhang F-J, Yu L-N, Xing T, et al. Mild volume acute normovolemic hemodilution is associated with lower intraoperative transfusion and postoperative pulmonary infection in patients undergoing cardiac surgery -- A retrospective, propensity matching study. BMC Anesthesiol 2017;17:13.  Back to cited text no. 6
Goldberg J, Paugh TA, Dickinson TA, Fuller J, Paone G, Theurer PF, et al. Greater volume of acute normovolemic hemodilution may aid in reducing blood transfusions after cardiac surgery. Ann Thorac Surg. 2015;100:1581-7.  Back to cited text no. 7
Kochamba GS, Pfeffer TA, Sintek CF, Khonsari S. Intraoperative autotransfusion reduces blood loss after cardiopulmonary bypass. Ann Thorac Surg 1996;61:900–3.  Back to cited text no. 8
Helm RE, Klemperer JD, Rosengart TK, Gold JP, Peterson P, DeBois W, et al. Intraoperative autologous blood donation preserves red cell mass but does not decrease postoperative bleeding. Ann Thorac Surg 1996;62:1431–41.  Back to cited text no. 9
Bryson GL, Laupacis A, Wells GA. Does acute normovolemic hemodilution reduce perioperative allogeneic transfusion? A meta-analysis. The international study of perioperative transfusion. Anesth Analg 1998;86:9–15.  Back to cited text no. 10
Barile L, Fominskiy E, Di Tomasso N, Alpizar Castro LE, Landoni G, De Luca M, et al. Acute normovolemic hemodilution reduces allogeneic red blood cell transfusion in cardiac surgery: A systematic review and meta-analysis of randomized trials. Anesth Analg 2017;124:743-52.  Back to cited text no. 11
Höhn L, Schweizer A, Licker M, Morel DR. Absence of beneficial effect of acute normovolemic hemodilution combined with aprotinin on allogeneic blood transfusion requirements in cardiac surgery. Anesthesiology 2002;96:276–82.  Back to cited text no. 12
Casati V, Speziali G, D'Alessandro C, Cianchi C, Grasso MA, Spagnolo S, et al. Intraoperative low-volume acute normovolemic hemodilution in adult open-heart surgery. Anesthesiology 2002;97:367–73.  Back to cited text no. 13
Hyde JA, Chinn JA, Graham TR. Platelets and cardiopulmonary bypass. Perfusion 1998;13:389-407.  Back to cited text no. 14
Ramnarine IR, Higgins MJ, McGarrity A, Mahmood Z, Wheatley DJ, Belcher PR. Autologous blood transfusion for cardiopulmonary bypass: Effects of storage conditions on platelet function. J Cardiothorac Vasc Anesth 2006;20:541-7.  Back to cited text no. 15
Zisman E, Eden A, Shenderey A, Meyer G, Balagula M, Ammar R, et al. The effect of acute autologous blood transfusion on coagulation dysfunction after cardiopulmonary bypass. Eur J Anaesthesiol 2009;26:868-73.  Back to cited text no. 16
Flom-Halvorsen HI, Ovrum E, Oystese R, Brosstad F. Quality of intraoperative autologous blood withdrawal used for retransfusion after cardiopulmonary bypass. Ann Thorac Surg 2003;76:744-8.  Back to cited text no. 17
Saad H, Aladawy M. Temperature management in cardiac surgery. Glob Cardiol Sci Pract 2013;2013:44-62.  Back to cited text no. 18
Lamke LO, Liljedahl SO. Plasma volume changes after infusion of various plasma expanders. Resuscitation 1976;5:93-102.  Back to cited text no. 19
SAFE Study Investigators; Finfer S, McEvoy S, Bellomo R, McArthur C, Myburgh J, et al. Impact of albumin compared to saline on organ function and mortality of patients with severe sepsis. Intensive Care Med 2011;37:86-96.  Back to cited text no. 20
Rasmussen SR, Kandler K, Nielsen RV, Jakobsen PC, Ranucci M, Ravn HB. Association between transfusion of blood products and acute kidney injury following cardiac surgery. Acta Anaesthesiol Scand 2020;64:1397-404.  Back to cited text no. 21
Zimmermann E, Zhu R, Ogami T, Lamonica A, Petrie JA 3rd, Mack C, et al. Intraoperative autologous blood donation leads to fewer transfusions in cardiac surgery. Ann Thorac Surg 2019;108:1738-44.  Back to cited text no. 22
van der Wal MT, Boks RH, Wijers-Hille MJ, Hofland J, Takkenberg JJ, Bogers AJ. The effect of pre-operative blood withdrawal, with or without sequestration, on allogeneic blood product requirements. Perfusion 2015;30:643-9.  Back to cited text no. 23
Ramnath AN, Naber HR, de Boer A, Leusink JA. No benefit of intraoperative whole blood sequestration and autotransfusion during coronary artery bypass grafting: Results of a randomized clinical trial. J Thorac Cardiovasc Surg 2003;125:1432-7.  Back to cited text no. 24
Mehta RH, Castelvecchio S, Ballotta A, Frigiola A, Bossone E, Ranucci M. Association of gender and lowest hematocrit on cardiopulmonary bypass with acute kidney injury and operative mortality in patients undergoing cardiac surgery. Ann Thorac Surg 2013;96:133-40.  Back to cited text no. 25
Wuethrich PY, Studer UE, Thalmann GN, Burkhard FC. Intraoperative continuous norepinephrine infusion combined with restrictive deferred hydration significantly reduces the need for blood transfusion in patients undergoing open radical cystectomy: Results of a prospective randomised trial. Eur Urol 2014;66:352-60.  Back to cited text no. 26

Correspondence Address:
Milo Engoren
Department of Anesthesiology, University of Michigan, 1500 E. Medical Center Drive, Ann Arbor, MI - 48106
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/aca.aca_30_21

Rights and Permissions


  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5]